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pzt c13 l1 jizhong zou addgene  (Addgene inc)


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    Structured Review

    Addgene inc pzt c13 l1 jizhong zou addgene
    Pzt C13 L1 Jizhong Zou Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/jizhong+zou/pZT-C13-R1+(Plasmid+%2362197)/pm41610849-345-215-218
    Average 94 stars, based on 32 article reviews
    pzt c13 l1 jizhong zou addgene - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Synthesized:

    Article Title: High-throughput screening for small-molecule stabilizers of misfolded glucocerebrosidase in Gaucher disease and Parkinson’s disease
    Article Snippet: .. The gRNA targeting LIMP-2 (acagttgacgaattgctctg) was synthesized by Integrated DNA Technologies and cloned into pCAG-eCas9-GFP-U6-gRNA, an all-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9, to generate pCAG-eCas9-GFP-U6-LIMP2_gRNA. pCAG-eCas9GFP-U6-gRNA was a gift from Jizhong Zou (Addgene plasmid # 79145; http://n2t.net/addgene:79145; RRID:Addgene_79145). pCAG-eCas9-GFP-U6-LIMP2_gRNA was transfected into GBA1-WT and HiBiT-hGCase-WT H4 cells. ..

    Clone Assay:

    Article Title: High-throughput screening for small-molecule stabilizers of misfolded glucocerebrosidase in Gaucher disease and Parkinson’s disease
    Article Snippet: .. The gRNA targeting LIMP-2 (acagttgacgaattgctctg) was synthesized by Integrated DNA Technologies and cloned into pCAG-eCas9-GFP-U6-gRNA, an all-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9, to generate pCAG-eCas9-GFP-U6-LIMP2_gRNA. pCAG-eCas9GFP-U6-gRNA was a gift from Jizhong Zou (Addgene plasmid # 79145; http://n2t.net/addgene:79145; RRID:Addgene_79145). pCAG-eCas9-GFP-U6-LIMP2_gRNA was transfected into GBA1-WT and HiBiT-hGCase-WT H4 cells. ..

    Article Title: Combining light-induced aggregation and biotin proximity labeling implicates endolysosomal proteins in early α-synuclein oligomerization
    Article Snippet: The donor plasmid pC13N-iCAG.copGFP was a gift from Jizhong Zou (Addgene, Cat. 66578). .. Using this plasmid, we cloned in LIPA-α-syn to create the iCAG-LIPA-α-syn transgene, flanked by homology arms corresponding to the CLYBL locus, this plasmid was co-transfected with TALEN constructs: pZT-C13-L1, and pZT-C13-R1, these plasmids were a gift from Jizhong Zou (Addgene, Cat. 62196; 62197). .. We used the Nucleofector X Solution (Lonza, Cat.V4XP-3024) to prepare the transfection mix, which contained 5 μg of each TALEN construct and 10 μg of the donor plasmid.

    Article Title: Combining light-induced aggregation and biotin proximity labeling strongly implicates endolysosomal proteins in early α-synuclein oligomerization
    Article Snippet: The donor plasmid pC13N-iCAG.copGFP was a gift from Jizhong Zou (Addgene, Cat. 66578)108. .. Using this plasmid, we cloned in LIPA-α-syn to create the iCAG-LIPA-α-syn transgene, flanked by homology arms corresponding to the CLYBL locus, this plasmid was co-transfected with TALEN constructs: pZTC13-L1, and pZT-C13-R1, these plasmids were a gift from Jizhong Zou (Addgene, Cat. 62196; 62197)108. .. We used the Nucleofector X Solution (Lonza, Cat.V4XP-3024) to prepare the transfection mix, which contained 5 μg of each TALEN construct and 10 μg of the donor plasmid.

    CRISPR:

    Article Title: High-throughput screening for small-molecule stabilizers of misfolded glucocerebrosidase in Gaucher disease and Parkinson’s disease
    Article Snippet: .. The gRNA targeting LIMP-2 (acagttgacgaattgctctg) was synthesized by Integrated DNA Technologies and cloned into pCAG-eCas9-GFP-U6-gRNA, an all-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9, to generate pCAG-eCas9-GFP-U6-LIMP2_gRNA. pCAG-eCas9GFP-U6-gRNA was a gift from Jizhong Zou (Addgene plasmid # 79145; http://n2t.net/addgene:79145; RRID:Addgene_79145). pCAG-eCas9-GFP-U6-LIMP2_gRNA was transfected into GBA1-WT and HiBiT-hGCase-WT H4 cells. ..

    Plasmid Preparation:

    Article Title: High-throughput screening for small-molecule stabilizers of misfolded glucocerebrosidase in Gaucher disease and Parkinson’s disease
    Article Snippet: .. The gRNA targeting LIMP-2 (acagttgacgaattgctctg) was synthesized by Integrated DNA Technologies and cloned into pCAG-eCas9-GFP-U6-gRNA, an all-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9, to generate pCAG-eCas9-GFP-U6-LIMP2_gRNA. pCAG-eCas9GFP-U6-gRNA was a gift from Jizhong Zou (Addgene plasmid # 79145; http://n2t.net/addgene:79145; RRID:Addgene_79145). pCAG-eCas9-GFP-U6-LIMP2_gRNA was transfected into GBA1-WT and HiBiT-hGCase-WT H4 cells. ..

    Article Title: Combining light-induced aggregation and biotin proximity labeling strongly implicates endolysosomal proteins in early α-synuclein oligomerization
    Article Snippet: .. The donor plasmid pC13N-iCAG.copGFP was a gift from Jizhong Zou (Addgene, Cat. 66578)108. .. Using this plasmid, we cloned in LIPA-α-syn to create the iCAG-LIPA-α-syn transgene, flanked by homology arms corresponding to the CLYBL locus, this plasmid was co-transfected with TALEN constructs: pZTC13-L1, and pZT-C13-R1, these plasmids were a gift from Jizhong Zou (Addgene, Cat. 62196; 62197)108.

    Article Title: Efficient in vivo labeling of endogenous proteins with SMART delineates retina cellular and synaptic organization
    Article Snippet: The pCAG-GFP plasmid was a gift from Connie Cepko (Addgene plasmid # 11150) . .. The pCAG-eCas9-GFP-U6-gRNA was a gift from Jizhong Zou (Addgene plasmid # 79145). ..

    Article Title: Combining light-induced aggregation and biotin proximity labeling implicates endolysosomal proteins in early α-synuclein oligomerization
    Article Snippet: .. The donor plasmid pC13N-iCAG.copGFP was a gift from Jizhong Zou (Addgene, Cat. 66578). .. Using this plasmid, we cloned in LIPA-α-syn to create the iCAG-LIPA-α-syn transgene, flanked by homology arms corresponding to the CLYBL locus, this plasmid was co-transfected with TALEN constructs: pZT-C13-L1, and pZT-C13-R1, these plasmids were a gift from Jizhong Zou (Addgene, Cat. 62196; 62197).

    Article Title: Efficient in vivo labeling of endogenous proteins with SMART delineates retina cellular and synaptic organization.
    Article Snippet: The pCAG-GFP plasmid was a gift from Connie Cepko (Addgene plasmid # 11150)14. .. The pCAG-eCas9-GFP-U6-gRNA was a gift from Jizhong Zou (Addgene plasmid # 79145). ..

    Article Title: Combining light-induced aggregation and biotin proximity labeling implicates endolysosomal proteins in early α-synuclein oligomerization
    Article Snippet: The donor plasmid pC13N-iCAG.copGFP was a gift from Jizhong Zou (Addgene, Cat. 66578). .. Using this plasmid, we cloned in LIPA-α-syn to create the iCAG-LIPA-α-syn transgene, flanked by homology arms corresponding to the CLYBL locus, this plasmid was co-transfected with TALEN constructs: pZT-C13-L1, and pZT-C13-R1, these plasmids were a gift from Jizhong Zou (Addgene, Cat. 62196; 62197). .. We used the Nucleofector X Solution (Lonza, Cat.V4XP-3024) to prepare the transfection mix, which contained 5 μg of each TALEN construct and 10 μg of the donor plasmid.

    Article Title: Combining light-induced aggregation and biotin proximity labeling strongly implicates endolysosomal proteins in early α-synuclein oligomerization
    Article Snippet: The donor plasmid pC13N-iCAG.copGFP was a gift from Jizhong Zou (Addgene, Cat. 66578)108. .. Using this plasmid, we cloned in LIPA-α-syn to create the iCAG-LIPA-α-syn transgene, flanked by homology arms corresponding to the CLYBL locus, this plasmid was co-transfected with TALEN constructs: pZTC13-L1, and pZT-C13-R1, these plasmids were a gift from Jizhong Zou (Addgene, Cat. 62196; 62197)108. .. We used the Nucleofector X Solution (Lonza, Cat.V4XP-3024) to prepare the transfection mix, which contained 5 μg of each TALEN construct and 10 μg of the donor plasmid.

    Article Title: Regulation potential of transcribed simple repeated sequences in developing neurons.
    Article Snippet: Tissue culture (TC) plates used for culturing iPSCs (typically Thermo Fisher Scientific cat# 140675) were coated by incubating them with 1 μg/cm2 of vitronectin (Thermo Fisher Scientific, cat# A14700) at room temperature for 1 h. For normal passaging, iPSC colonies were incubated with 0.5 mM EDTA (Thermo Fisher Scientific, cat# 15575020) in DPBS (no calcium, no magnesium; Thermo Fisher Scientific, cat# 14190094) at room temperature for 4–8 min and then gently dissociated by trituration in the growth medium. .. The pZT-C13-L1 and pZT-C13-R1 constructs encoding the left and right TALENs specific to the CLYBL safe-harbor locus were a gift from Jizhong Zou (Addgene plasmid #62196 and #62197; Cerbini et al. 2015). .. The CLYBL-specific homology directed repair construct pUCM-CLYBLhNIL was a gift from Michael Ward (Addgene plasmid #105841; Fernandopulle et al. 2018).

    Transfection:

    Article Title: High-throughput screening for small-molecule stabilizers of misfolded glucocerebrosidase in Gaucher disease and Parkinson’s disease
    Article Snippet: .. The gRNA targeting LIMP-2 (acagttgacgaattgctctg) was synthesized by Integrated DNA Technologies and cloned into pCAG-eCas9-GFP-U6-gRNA, an all-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9, to generate pCAG-eCas9-GFP-U6-LIMP2_gRNA. pCAG-eCas9GFP-U6-gRNA was a gift from Jizhong Zou (Addgene plasmid # 79145; http://n2t.net/addgene:79145; RRID:Addgene_79145). pCAG-eCas9-GFP-U6-LIMP2_gRNA was transfected into GBA1-WT and HiBiT-hGCase-WT H4 cells. ..

    Construct:

    Article Title: Combining light-induced aggregation and biotin proximity labeling implicates endolysosomal proteins in early α-synuclein oligomerization
    Article Snippet: The donor plasmid pC13N-iCAG.copGFP was a gift from Jizhong Zou (Addgene, Cat. 66578). .. Using this plasmid, we cloned in LIPA-α-syn to create the iCAG-LIPA-α-syn transgene, flanked by homology arms corresponding to the CLYBL locus, this plasmid was co-transfected with TALEN constructs: pZT-C13-L1, and pZT-C13-R1, these plasmids were a gift from Jizhong Zou (Addgene, Cat. 62196; 62197). .. We used the Nucleofector X Solution (Lonza, Cat.V4XP-3024) to prepare the transfection mix, which contained 5 μg of each TALEN construct and 10 μg of the donor plasmid.

    Article Title: Combining light-induced aggregation and biotin proximity labeling strongly implicates endolysosomal proteins in early α-synuclein oligomerization
    Article Snippet: The donor plasmid pC13N-iCAG.copGFP was a gift from Jizhong Zou (Addgene, Cat. 66578)108. .. Using this plasmid, we cloned in LIPA-α-syn to create the iCAG-LIPA-α-syn transgene, flanked by homology arms corresponding to the CLYBL locus, this plasmid was co-transfected with TALEN constructs: pZTC13-L1, and pZT-C13-R1, these plasmids were a gift from Jizhong Zou (Addgene, Cat. 62196; 62197)108. .. We used the Nucleofector X Solution (Lonza, Cat.V4XP-3024) to prepare the transfection mix, which contained 5 μg of each TALEN construct and 10 μg of the donor plasmid.

    Article Title: Regulation potential of transcribed simple repeated sequences in developing neurons.
    Article Snippet: Tissue culture (TC) plates used for culturing iPSCs (typically Thermo Fisher Scientific cat# 140675) were coated by incubating them with 1 μg/cm2 of vitronectin (Thermo Fisher Scientific, cat# A14700) at room temperature for 1 h. For normal passaging, iPSC colonies were incubated with 0.5 mM EDTA (Thermo Fisher Scientific, cat# 15575020) in DPBS (no calcium, no magnesium; Thermo Fisher Scientific, cat# 14190094) at room temperature for 4–8 min and then gently dissociated by trituration in the growth medium. .. The pZT-C13-L1 and pZT-C13-R1 constructs encoding the left and right TALENs specific to the CLYBL safe-harbor locus were a gift from Jizhong Zou (Addgene plasmid #62196 and #62197; Cerbini et al. 2015). .. The CLYBL-specific homology directed repair construct pUCM-CLYBLhNIL was a gift from Michael Ward (Addgene plasmid #105841; Fernandopulle et al. 2018).

    TALENs:

    Article Title: Regulation potential of transcribed simple repeated sequences in developing neurons.
    Article Snippet: Tissue culture (TC) plates used for culturing iPSCs (typically Thermo Fisher Scientific cat# 140675) were coated by incubating them with 1 μg/cm2 of vitronectin (Thermo Fisher Scientific, cat# A14700) at room temperature for 1 h. For normal passaging, iPSC colonies were incubated with 0.5 mM EDTA (Thermo Fisher Scientific, cat# 15575020) in DPBS (no calcium, no magnesium; Thermo Fisher Scientific, cat# 14190094) at room temperature for 4–8 min and then gently dissociated by trituration in the growth medium. .. The pZT-C13-L1 and pZT-C13-R1 constructs encoding the left and right TALENs specific to the CLYBL safe-harbor locus were a gift from Jizhong Zou (Addgene plasmid #62196 and #62197; Cerbini et al. 2015). .. The CLYBL-specific homology directed repair construct pUCM-CLYBLhNIL was a gift from Michael Ward (Addgene plasmid #105841; Fernandopulle et al. 2018).



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